The first step in getting started with darkfield microscopy is to make sure that the system one is acquiring is configured to one's needs. Once deciding on the model and camera, one needs a few basic supplies and usually some assistance in setting up the system and learning to use it. Mistakes can be costly so it pays to have some help with the assembly and proper use of the equipment.
Next, of course, comes the sampling and there are some tips for preparing the slides and coverslips as well as how to take the best sample possible. Then, the sample is placed on the microscope stage and explored. Depending on one's background, what one does and does not actually observe can differ. Being a medical doctor, hemotologist, pathologist, or microscopist does not necessarily prepare one for interpreting what is seen in darkfield.
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ZETA POTENTIAL
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METAL TOXICITY
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BLOOD PARASITES
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White Blood Cells
This patient recovered fully from a very serious health condition once the amalgam dental fillings were replaced.
Rouleaux can occur for many reasons: dehydration being the most common. However, sometimes the red blood cells get snagged on fibrin . . . as in this case.
Thrombosis can occur when many cells are tightly packed. This often occurs with severe dehydration such as high altitude flying and sitting for hours without much movement. It can happen for other reasons such as cells sticking together because of damage to the surface membranes . . . which may, in turn, be due to chemical toxicity or very low zeta potential.
There are many types of blood parasites. Some are intraerythrocytic, meaning they live mainly inside red blood cells. However, when they mature, the red blood cells rupture and cause symptoms such as are associated with malaria and babesia. Other parasites live mainly in the plasma and forage on red blood cells. There are about 4000 known types of parasites that affect humans.
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When so many white blood cells are found together, it is usually because there is a problem. They work as a group to resolve the issue, sometimes perishing in the process.
